Fig. 4

Caspase-independent pathway of apoptosis does not interfere with cell fate upon MB-PDT. (a) WB analysis of BCL2/BAX ratio in MDA-MB-231, MCF-7 and MCF-10A cells subjected or not to MB-PDT upon 1 h, 3 h or 24 h post-irradiation. Immunoblots shown are representative results. The corresponding bar graph results from densitometry analysis from all blots. Results are presented as mean ± s.e.m., (n = 3 independent experiments); * p < 0.05 versus control. (b) Viability time curves obtained after 1 h, 3 h and 24 h post MB-PDT in the presence or absence of a specific pharmacological BAX inhibitor (n = 3 independent experiments) * p < 0.05 versus MB-PDT. Results are shown as mean ± s.e.m