Fig. 2

Cachexia induces overexpression of collagen type I, III, VI and fibronectin in the adipose tissue. a-d Sections of human subcutaneous adipose tissue were immunostained with (a) COL1A1, (b) COL3A1, (c) COL6A1, and (d) Fibronectin (FN1). Control (n = 5), Weight-stable cancer (WSC; n = 5), Cancer cachexia (CC; n = 5). Note the positive labeling for all groups, with stronger intensity in CC. Immunostaining for COL6A1 in Control, WSC and CC samples was found around the adipocytes, whereas, in CC, positive staining in several fibrotic areas were observed (F). Ad- Illustrates preserved adipocytes. Blue staining (Mayer’s Haematoxylin) represents a non-reactive nucleous