Fig. 6

Cec-induced cell death in NSG tumors. Tumors at week 6 were removed. H&E staining was performed in a treated tumor (a) and a control tumor (b). c Percentage of dead cells in control and treat mice. Tumors at week 3.5 were removed. Live/dead NBT (nitro blue tetrazolium) staining was performed in a treated tumor (d) and in a control tumor (e). Yellow dash line indicates live (a) or dead cells (b, d). f Percent growth of treated and control tumors. * indicates statistical significance in comparison to the control tumor. g The pro-caspase was detected in MDA-MB-231 cells near 36 kDa (N for non-transfection). The active caspase appeared around 20 kDa in cells expressing Cec but not GFP (another negative control) or non-transfection (N). GAPDH was used as the loading controls. Cleaved caspase-3 expression (cells in brown color) is shown in untreated small tumor (yellow arrows) (h) and in Cec-treated small tumor (i), respectively. j Percentage of caspase-3 expression cells in untreated or treated with empty lentiviral vector and Cec-treated small tumors. * indicates statistical significance comparing to untreated or GFP treated tumor