Fig. 1
From: Altering calcium influx for selective destruction of breast tumor
Ca2+ influx through L-type voltage-gated calcium channels in breast cancer cells. a Lack of voltage-gated calcium currents in response to the 500 ms depolarizing pulses from -50 mV to +50 mV in 10mv increments (inset). b Representative T-type calcium current in a MCF7 cell (inset is the pulse protocol). c After 3-day transfection of Cav1.2 in MCF7 cells, L-type calcium current was elicited by a 500 ms depolarizing pulse to -10 mV; gray lines show the Ca2+ influx. d After 1-day transfection of Cec, L-type calcium current was elicited by a 500 ms depolarizing pulse to -10 mV. Gray lines in the enclosed area in b, c, and d indicate the Ca2+ influx. e Normalized current–voltage (IV) relationship of L-type Cav1.2 (ICaL) and Cec expressed in MCF7 cells (n = 6 in each group), the membrane potential was held at -80 mV. f Ca2+ influx generated by T-type calcium current, L-type calcium current, and Cec calcium current in response to a 500 ms depolarizing pulse to -10 mV, respectively (n = 6). * indicates statistical significance compare to ICa in MCF7 and in HMEC groups. The membrane potential was held at -80 mV