Fig. 7

Glioblastoma xenograft. Tumor xenografts were generated by injecting tumor cells into the brains of nude Balb/c mice. The mice received 0.5% methylcellulose in 0.9% NaCl (control) or siramesine. After the experiment, the mice brains were fixed, paraffin embedded, sectioned (3 μm) and immunohistochemically stained with anti-human specific CD56 in order to identify the tumor cells. a–b, e The standard U87 cell line formed fast growing circumscribed tumors. Siramesine treatment was initiated 1 week after tumor implantation and sustained for 1 week but did not reduce tumor volume (P = 0.78). c–d, f Patient-derived T78 spheroids formed slower growing invasive tumors. Siramesine treatment was initiated 2 weeks after tumor implantation and sustained for a total of 6 weeks but did not reduce tumor volume (P = 0.62). Scalebar 2 mm (a–d). Data are displayed as mean values ± SEM, statistical significance was assessed students t-test. AU, arbitraty units