Fig. 3
From: Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
Flat surface spheroid migration assay. The glioblastoma stem cell-like containing spheroid (GSS) culture T78 and T86 (images not shown) were allowed to migrate for 1 day before exposure to siramesine (0–20 μM). a, e, i, m, q Control spheroids revealed a pronounced migration. Siramesine reduced migration in T78 spheroids at all concentrations; b, f, j, n, r 5 μM, c, g, k, o, s 10 μM, and d, h, l, p, t 20 μM. u The spheroid diameter including all migrating cells was measured at indicated time-points. T78 spheroids exposed to 20 μM siramesine showed a significantly reduced migration distance already 1 day after exposure (in total 48 h after start of experiment), compared to control spheroids. v The migration distance was significantly reduced in T86 spheroids 2 days after siramesine exposure (in total 72 h after start of experiment) at all concentrations. Control cells received culture medium or DMSO (images not shown) both without siramesine. Scalebar 100 μm (a–t). Data are displayed as mean values ± SEM, and **P < 0.01, ***P < 0.001 were assessed by one-way ANOVA