Fig. 3

MiR-155 targets KRAS. a Schematic representation of KRAS 3’ UTR and relative position of the predicted miRNA- binding site. b Western blot analysis of KRAS protein levels in HEK-293 cells transfected with miR-155 or a scrambled oligonucleotide. Actin expression was analyzed as loading control. A representative experiment is shown. c qRT-PCR analyses of KRAS mRNA expression in HEK-293 cells transfected with miR-155 or a scrambled oligonucleotide, normalized with G6PD. qRT-PCR analysis was performed in triplicate and reported values represent the mean ± SD. d Relative luciferase activity in HEK-293 cells transiently transfected with 3’UTR-KRAS and 3’UTR-KRAS mutated in the miR-155 seed sequence along with the miR 155 or with a scrambled oligonuclotide. e Western blot analysis of KRAS protein in MEFs Cbx7^+/+ (WT) and Cbx7^-/- (KO). Gapdh expression was analyzed as loading control. **, P < 0.01 (t test)