Fig. 4

The CHOP binding domain is critical for CPA-activated DR5 gene promoter activity. DU145 cells were transfected with the luciferase reporter plasmids containing various lengths of the DR5 gene promoter. Cells were treated without or with 50 μM CPA for 24 h and then subjected to luciferase activity assays. a Upper panel, schematic structure of reporter plasmids; lower panel, statistical analysis of three independent luciferase assay results. Data shown are means ± S.E. with **p < 0.01, ns: not significant. The relative luciferase activity of DR5-P(−711/+3) without CPA treatment was set as one. b Upper panel, schematic structure of DR5 promoter reporter plasmids containing wide-type or mutated CHOP binding site; lower panel, statistical analysis of three independent luciferase assay results. Data shown are means ± S.E. with **p < 0.01, ns: not significant. The relative luciferase activity of DR5-P(−552/+3) without CPA treatment was set as one