Fig. 5From: A novel FOXO1-mediated dedifferentiation blocking role for DKK3 in adrenocortical carcinogenesisFOXO1 silencing releases DKK3-mediated block of cell migration. Cells were treated with scrambled negative siRNA (Neg.) or FOXO1 siRNA (Si-RNA) for 24Â h, trypsinized, and allowed to migrate for 4Â h through modified Boyden chamber. Migrated cells were fixed, stained, and counted manually. Total number of SW-Neo cells treated with scrambled siRNA normalized to 100%, and relative change in migration of SW-Neo and SW-DKK3 cells treated with FOXO1 siRNA is shown in overlapping line graph on left Y-axis. Relative FOXO1 expression in SW-Neo and SW-DKK3 cells treated with FOXO1 siRNA, normalized to FOXO1 expression in SW-Neo cells treated with scrambled siRNA set at 100 shown as bars on right Y-axisBack to article page