Fig. 4
The influence of NF-κB inhibitor wogonin on LMP1/miR-155/PU.1 in Raji cells. a Flow cytometry was used to detect the apoptosis of Raji cells after treated with PDTC at 25μmol/L for 48 h. The ratio of apoptosis was 8.5 ± 0.74% and 10.19 ± 1.71% for the control and PDTC-treated groups respectively. b Effect of PDTC and PDTC with wogonin on the mRNA expression of LMP1, miR-155 and PU.1 of Raji cells detected by PCR and analyzed by the ΔCt method after treated at the concentration of 25 μmol/L PDTC, 50μmol/L wogonin and their combination for 48 h. Bars were shown as mean ± SD (n = 3) and represent LMP1/GAPDH or PU.1/GAPDH or miR-155/U6 ratios relative to the control group (*P < 0.05). c The protein expression of LMP1 and PU.1 were analyzed by western blots to evaluate the effect of PDTC (25 μmol/L), wogonin (50μmol/L) and their combination for 48 h in Raji cells. GAPDH was used as a loading control. d For quantity of (c), images were analyzed using Image J. Bars were the mean ± SD (n = 3). e Effect of wogonin on the NF-κB transcriptional activation was evaluated by Dual-luciferase reporter assay in wogonin-treated Raji cells. The comparisons were made relative to control group, and the difference levels of significance was indicated as (*P < 0.05 and **P < 0.01)