Fig. 4

MT1H regulates Wnt/β-catenin signaling in HCC cells. a GSEA analysis showed that MT1H might has a regulative role in Wnt/β-catenin signaling in HCC cells. b qRT-PCR analysis was used to detect the mRNA levels of MYC, MMP7, and LEF1 in indicated cells. Error bars indicate standard deviation (Student’s t-test; *, P < 0.05). c Wnt/β-catenin signaling activity was assessed by TOP/FOP flash dual-luciferase reporter assay in indicated cells. Error bars indicate standard deviation (Student’s t-test; *, P < 0.05). d Immunofluorescence of β-catenin distribution in indicated cells. e Western blot analysis of nuclear extraction from indicated cells. f Western blot analysis was used to detected the level of p-Akt (Ser 473), total-Akt, p-GSK-3β (Ser 9), and total-GSK-3β in indicated cells. α-Tubulin as a loading control