Fig. 2

MT1H plays a pivotal role in HCC cell proliferation. a Expression of MT1H-Flag in indicated cells. Protein extracts from indicated cells were immunoblotted with antibody for Flag. α-Tubulin as a loading control. b HepG2-Vector, HepG2-MT1H, Hep3B-Vector, and HepG3B-MT1H cells was measured by MTT response. Error bars indicate standard deviation (Student’s t-test; *, P < 0.05). c Colony number of MT1H overexpressing HepG2 and Hep3B cells was significantly decreased as compared with respective vector-control. Error bars indicate standard deviation (Student’s t-test; *, P < 0.05). d Edu incorporation analysis indicates that MT1H suppresses the proliferation of HCC cells. Error bars indicate standard deviation (Student’s t-test; *, P < 0.05)