Fig. 6

Immunohistochemical analysis in testis and benign prostate tissues for TSPYL5 protein expression. a Normal human testis showing seminiferous tubules with normal spermatogenesis (400×) (b) TSPYL5 protein expression in human testis tissue (positive control) which exhibited membrane, nuclear and cytoplasmic protein staining (400×). c Benign prostate tissues showing benign acini lined by inner secretory epithelial cells and outer basal cell layer (400×). d Benign prostate tissues exhibited more cytoplasmic membrane TSPYL5 protein staining (400×). e Prostate adenocarcinoma Gleason pattern 3 with small glands (400×). f Tumor tissues with GS-6 (3 + 3) exhibited both nuclear and cytoplasmic staining with a composite score (CS) of 12 (3x4) (400×). g Prostate adenocarcinoma Gleason pattern 4 with cribriform glands (400×). h Prostate adenocarcinoma with GS-7 (3 + 4), show moderate cytoplasmic and membrane staining with CS of 8 (2×4) (400×). i Prostate adenocarcinoma with Gleason score 4 (200×). j In the tumor specimens with GS-8 (4 + 4) or above the staining intensity was very low and confined to the membranes (CS = ≤1) with no nuclear or cytoplasmic staining (400×). The depicted images are representative of three cases examined individually in tissues with various GS. Arrows in the figure denotes TSPYL5 protein expression. (H & E-a, c, e, g, i; Immuno – b, d, f, h, j). The protein staining intensity between benign and tumor tissues with GS −8 (P = 0.012) and GS-7 (P = 0.032) were significant, while no significant difference was observed in staining intensities between benign and tumor tissues with GS-6 (P > 0.05)