Fig. 5

TSPYL5 mRNA expression in tissues and MSP analysis. PCR analysis of TSPYL5 mRNA expression in (a) benign, and b tumor tissues. Variable TSPYL5 mRNA expression was observed in benign as well as tumor samples with insignificant expression in tumor tissues with GS ≥ 8. c A representative MSP results from benign and tumor samples (denoted above in the gels (a and b) by B# or T#). Low or moderate methylation alleles was observed in benign and tumor tissues with GS-6 or-7, while strong methylated bands was observed in tumor tissues with GS ≥ 8. Densitometry gel analysis indicated a decrease in the TSPYL5 mRNA expression in tissues with GS-7 (P = 0.012) and GS ≥ 8 (P = 0.001). PCR with unmethylated primers indicate the presence of unmethylated bands in benign and tumor tissues with GS-6 or −7, but relatively lower in tumor tissues with GS ≥ 8