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Fig. 4 | BMC Cancer

Fig. 4

From: Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J

Fig. 4

Knockdown of CCNJ enhanced the sensitivity to DDP in A549/DDP and SPC-A1/DDP cells. a The mRNA levels of CCNJ in A549/DDP and SPC-A1/DDP cells transfected with siCCNJ or NC were analyzed by qRT-PCR. b The cell viability was evaluated by CCK-8 assay, A549/DDP and SPC-A1/DDP cells transfected with siCCNJ or NC treated with serially diluted DDP. c Representative data of FACS statistical graph analyses of cell cycle in A549/DDP and SPC-A1/DDP cells transfect with siCCNJ or NC for 6 h and then incubated with 1 μg/ml DDP for 48 h. d Representative data of FACS and statistical graph analyses of cell apoptosis in A549/DDP and SPC-A1/DDP cells transfected with siCCNJ or NC for 6 h and then incubated with 1 μg/ml DDP for 48 h. e Representative data of Hoechst staining assay in A549/DDP and SPC-A1/DDP cells transfected with siCCNJ or NC for 6 hand then incubated with 1 μg/ml DDP for 48 h. All data represented as mean value ± SD from 3 independent experiments. N.S. = no significant, * p < 0.05, **p < 0.01, ***p < 0.001

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