Fig. 1

Overexpression of miR-146a enhanced the sensitivity to DDP in A549/DDP and SPC-A1/DDP cells. a The mRNA levels of miR-146a in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus were analyzed by RT-qPCR. b The cell vitality was evaluated by CCK-8 assay, A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus treated with serially diluted DDP. c Representative data of FACS statistical graph analyses of cell cycle in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus and then treated with 1 μg/ml DDP for 48 h. d Representative data of FACS and statistical graph analyses of cell apoptosis in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus and incubated with 1 μg/ml DDP for 48 h. e Representative data of Hoechst staining assay in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus and then treated with 1 μg/ml DDP for 48 h. All data were expressed as mean value ± SD from 3 independent experiments. N.S. = no significant, *p < 0.05, **p < 0.01, ***p < 0.001