Fig. 8

AZ and/or MS-275 treatments affected hypoxia response and CAIX in NB xenograft cells. a-b present the IHC study (x20 and x40) on HIF1-α expression, (arrow) and the number of HIF-1α positive cells. c-d present CAIX expression (arrow) and the number of CAIX positive cells after 14 days treatment with AZ and/or MS-275 compared to untreated group in SH-SY5Y xenografts. HIF-1α expression was significantly reduced by AZ (29 ± 0.7%; p < 0.001), MS-275 (66 ± 0.23%; p < 0.001) and further enhanced in AZ + MS-275 (83 ± 0.67%; p < 0.001). CAIX expression was significantly reduced by AZ (18 ± 0.11%; p = 0.0138), MS-275 (73 ± 0.33%; p < 0.001) and markedly by AZ + MS275 (90 ± 0.16%; p < 0.001)