Fig. 7

AZ and/or MS-275 treatments affected the mitosis and proliferation of NB xenografts. a-b present the IHC study (x20 and x40) of the mitotic index (pHH3, arrow) and the number of pHH3 positive cells. c-d present the immunodetection and number of Ki67 positive cells (arrow) after 14 days treatment with AZ, MS-275 and AZ + MS-275 compared to untreated group in SH-SY5Y xenografts. Data shows that pHH3 expression was significantly reduced by AZ (42 ± 0.43%; p = 0.02), MS-275 (78 ± 0.16%; p = 0.002) and further by AZ + MS-275 (94 ± 0.05%; p = 0.001). Ki67 expression was moderately reduced by AZ (38 ± 0.70%; (p = 0.007), strongly reduced by MS-275 (70 ± 0.25%; p = 0.0002) and further reduced by AZ + MS-275 (84 ± 0.73%; p = 0.0001)