Fig. 3
From: Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma
a-b show the results of FACs analysis for 7-AAD and Annexin staining. Results demonstrate that compared to etoposide, MS-275 induces entry into early and late stage of apoptosis in 10.7% versus 2.51% and 7.66% versus 8.58% of cells, respectively. c show cleaved caspase 3 expression after 48 h treatment with 0.75 μM and 1.5 μM MS-275 that yielded 1% and 3% expression, respectively (p = 0.0003 and p < 0.0001, respectively as compared to control and p = 0.0001 when comparing doses). d-e show increased expression of apoptotic protein BAX and decreased BCL2/BAX ratio with coordinate decrease in survival following 0.75 μM and 1.5 μM MS-275 treatment to 15.59% and 14.86% of control. In addition, survivin expression reduced following 0.75 μM and 1.5 μM MS-275 treatment to 7.8% and 11%, respectively