Fig. 12

MS-275 treatment decreased expression of stem cell markers in NB and teratocarcinoma cells. a in the high OCT4, SOX2 and Nanog expressing NT2/D1 teratocarcinoma cell line, MS-275 was able to reduce nuclear expression of these stem cell markers as shown by immunofluorescence labeling. b Western blot analysis demonstrated a MS-275 reduction in expression of OCT4 and Nanog in NT2/D1, and OCT4 in SY5Y. c representative FACs profiles for OCT4 in SH-SY5Y and NT2/D1 cells. d calculated from FACs data in c. SH-SY5Y contains 0.38 ± 0.06% OCT4 positive and 0.33 ± 0.04% SOX2 positive cells. e MS-275 treatment significantly reduced expression of OCT4, SOX2 and Nanog in NT2/D1 at 0.75 μM (p = 0.0024, p < 0.0001 and p = 0.0031, respectively) and at 1.5 μM (p = 0.0096, p < 0.0001 and p = 0.0023, respectively). f when normalized to percentage of control, MS-275 treatment significantly reduced expression of OCT4 and SOX2 in SH-SY5Y at 0.75 μM (p = 0.0480 and p = 0.0391, respectively) and 1.5 μM (p = 0.0003 and p = 0.0066, respectively). g representative FACS profile for ABCG2 staining in SH-SY5Y and SK-N-BE(2) NB cell lines. h ABCG2 expression significantly decreases following MS-275 treatment in SH-SY5Y, SK-N-BE(2) and NT2/D1 cells at 0.75 μM (p = 0.0111, p = 0.0131 and p = 0.0086, respectively) and at 1.5 μM (p = 0.027, p = 0.0022 and p = 0.0084, respectively). Trypsin cleavage of cell surface ABCG2 was used as a negative control