Table 1 The primer sequences for PCRs
From: Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16
Primers | Sequence |
|---|---|
HPV16E6-F | 5’-GAGAACTGCAATGTTTCAGGACC-3’ |
HPV16E6-R | 5’-TGTATAGTTGTTTGCAGCTCTGTGC-3’ |
HPV16E2-F | 5’-AACGAAGTATCCTCTCCTGAAATTATTAG-3’ |
HPV16E2-R | 5’-CCAAGGCGACGGCTTTG-3’ |
HPV16MP-F | 5’-TGTAAAATTGTATATGGGTGTGTG-3’ |
HPV16MP-Rbio | 5’-(bio)ATCCTAAAACATTACAATTCTCTTTTAATA-3’ |
HPV16MP-S | 5’-TTTATGTATAAAATTAAGGG-3’ |
GAPDH-DF | 5’-TACTAGCGGTTTTACGGGCG-3’ |
GAPDH-DR | 5’-TCGAACAGGAGGAGCAGAGAGCGA-3’ |
GAPDH-cDF | 5’-GACAGTCAGCCGCATCTTCT-3’ |
GAPDH-cDR | 5’-TTAAAAGCAGCCCTGGTGAC-3’ |