Fig. 1

Isolation of tumour-associated glial cells. a GBM spheroids established from patient biopsies are implanted in GFP-NOD/scid mice. The resulting tumours are dissociated and GFP+ stromal cells are FACS- sorted with removal of CD31+ and CD11b + cells. b Flow cytometry histogram displays a bimodal curve, reflecting a non-fluorescent and a fluorescent cell population (Upper panel, Y-axis; cell count, X-axis; GFP fluorescence). Control fluorescence microscopy of cells sorted by GFP expression (middle panels) and after staining for human nuclear factor, a pan-human specific marker (HuNu, lower panels). c ICC for GFAP, Nestin, β-tubulin III and BrdU as indicated. Scale bar: 50 μm, insert: 20 μm. d Representative histograms (left panels) from cell cycle analysis of acutely isolated TAGs (n = 3, black) and control cells from normal mouse brain. (n = 3, white). Middle panel shows the percentage differences in S and G₂/M-phase between TAGs and control, while numbers for all cell cycle phases are outlined in the table (right)