Inhibition of glioblastoma dispersal by the MEK inhibitor PD0325901

BMC Cancer

Table 1 Tissue surface tension measurements and confirmation of liquidity for DMSO-treated and PD0325901 treated aggregates of primary GBM cells

Line	σ₁ dynes/cm ± s.e.m	σ₂ dynes/cm ± s.e.m	σ_1,2 dynes/cm ± s.e.m	t-test σ₁ vs σ₂ p	σ₂/σ₁	F₂/F₁	t-test σ₂/σ₁ vs F₂/F₁ p
GBM-1 DMSO	16.9 ± 1.9	19.4 ± 2.3	18.2 ± 1.5	0.4082	1.14 ± 0.05	1.36 ± 0.01	0.0003
GBM-1 PD03	18.4 ± 1.3	17.9 ± 1.2	18.1 ± 0.9	0.7951	0.98 ± 0.04	1.36 ± 0.02	<0.0001
GBM-2 DMSO	15.5 ± 1.4	16.9 ± 2.0	16.2 ± 1.2	0.5638	1.07 ± 0.08	1.35 ± 0.03	0.0072
GBM-2 PD03	15.2 ± 1.4	15.1 ± 1.0	15.2 ± 0.8	0.9035	1.02 ± 0.07	1.37 ± 0.04	0.0003
GBM-3 DMSO	8.5 ± 0.6	8.6 ± 0.7	8.6 ± 0.4	0.9041	1.01 ± 0.04	1.36 ± 0.02	<0.0001
GBM-3 PD03	8.9 ± 0.7	10.1 ± 0.6	9.5 ± 0.5	0.2389	1.15 ± 0.03	1.42 ± 0.07	0.0017
GBM-3 DMSO 300 FN	7.5 ± 0.7	7.1 ± 0.7	7.3 ± 0.5	0.6725	0.95 ± 0.03	1.28 ± 0.03	<0.0001
GBM-3 PD03 300 FN	32.7 ± 5.8	37.9 ± 6.6	35.3 ± 4.3	0.5550	1.17 ± 0.03	1.32 ± 0.03	0.0047
GBM-4 DMSO	16.2 ± 1.8	15.8 ± 1.2	16.0 ± 1.1	0.8833	1.01 ± 0.05	1.34 ± 0.01	<0.0001
GBM-4 PD03	20.6 ± 1.2	20.2 ± 1.0	20.4 ± 1.8	0.8312	1.02 ± 0.04	1.35 ± 0.02	<0.0001

For all cell lines, PD0325901 treatment did not result in a change in surface tension (pair-wise comparison by Student t-test, p > 0.05). GBM-3 was used to determine effects of exogenous fibronectin on surface tension. Here, addition of 300 μg/ml of soluble fibronectin resulted in a significant increase in aggregate surface tension (σ_1,2) of 7.3 ± 0.5 to 35.3 ± 4.3 dynes/cm (pairwise Student t-test, p < 0.0001). Liquid behavior was confirmed by demonstrating that 1) surface tension measured at two different compressions, the second greater than the first, were not statistically different, and 2) that the ratio of σ₂/σ₁ approaches 1 and is less than the ratio of the applied force at each successive compression (F2/F1)

ISSN: 1471-2407