Fig. 5

PD0325901 reduces growth rates of conventional 2D cultures and 3D spheroids of GBM cells. Growth rate for conventional 2D cultures of the GBM lines was measured either in DMSO (a) or PD0325901 (b). Fifty-thousand cells were plated and proliferation was monitored over a 4-day period. PD0325901 treatment significantly reduced the growth rate of 2D cultures. Aggregates (n = 6 for each line and treatment) of GBM-1 (c), GBM-2 (d), GBM-3 (e) and GBM-4 (f) were cultured either in the absence or presence of PD0325901 and area was measured for each aggregate once/day for 9 days. Linear regression was used to analyze the data. Only regression lines with an r² of 0.95 or higher were used. Regression lines depicted are average area as a function of time. Growth rate was significantly reduced by PD0325901 for all GBM lines as demonstrated by a significant shallowing of the slope of the line (ANCOVA, p < 0.0001). For GBM-1-4, growth rate was reduced 11.6, 2.7, 2.9, and 2.5-fold, respectively