Fig. 2

MPs increase the release of IL-6, TNF-α and INF-γ in THP-1 macrophages. THP-1 macrophage cells were co-cultured with 100 μg Res-MPs, Sen-MPs or D3-MPs for 4 h. Supernatants were collected and analysed using the Milliplex Human High Sensitivity T Cell magnetic panel-6-Plex Kit (Millipore, NSW, Australia) using luminex bead technology. a IL-6, b, IFN-γ and c TNF-α levels were analysed and mean ± SEM of at least 3 independent experiments conducted in duplicate are shown. Student’s unpaired two tailed T-test was used *P < 0.05, **P < 0.01, ***P < 0.001