Fig. 5

Functional Manipulation of SRF. a PC3-Age matched control (Ag) and PC3-docetaxel resistant (D12) cells for Western blotting analysis of SRF. β-actin was used as loading control. Fifty microgrammes of protein from untreated control (Ctrl), cells transfected with an empty vector; scramble control (Sc) and cells transfected with SRF siRNA knockdown (siRNA), were loaded into their respective wells. A representative image from three independent experiments is shown. SRF knockdown by siRNA was performed 48 h prior to treatment with 20 nM docetaxel for a further 48 h in 6 well plates seeded with ~100,000 cells per well of Ag and D12 cell lines respectively. b: Apoptosis was assessed using propidium iodide and flow cytometry (n = 3) and (c) Viability was assessed by MTT assay (n = 3). * = p < 0.05. ** = p < 0.01