Fig. 5

Levels of cytokines, IL-2, IFNγ and IL-5, produced in vitro. Levels of IL-2, IFNγ and IL-5 produced in vitro in splenocytes of mice immunized with different amounts of P467-CRM conjugate administered with Alum or Montanide, after re-stimulation with CRM (5a, 5b and 5c) or P467 (5d, 5e and 5f), are shown. Splenocytes of the immunized mice were used and re-stimulated, as described in materials and methods. The statistical analysis was performed using ANOVA (two-way) and linear contrasts. The measured levels of the controls (Alum Montanide and CRM) in Fig. 5d and f were lower than the background, and therefore were set to zero. Significant differences are denoted by asterisks. ns, not significant