Fig. 2
From: Mechanism of action of trabectedin in desmoplastic small round cell tumor cells
EWS-WT1 transcript heterogeneity in JN-DSRCT-1 cell line. a mRNA levels of EWS-WT1 chimera before and after 1 h of trabectedin 5 nM and 24 h after drug wash-out. Data are shown according to the Fold change method using PRISM GraphPad software.* p value < 0.005. b EWS-WT1 protein levels detected with antibodies against EWS (G-5, sc28327), WT1 (C-19, sc192), ACTIN (C-11, sc-1615). Western blot analysis of total extracts deriving from: lane 1, JN-DSRCT-1 cells untreated; lane 2, JN-DSRCT-1 cells 1hT48hR (1 h 5nM trabectedin treatment and 48 h after drug wash-out); lane 3, JN-DSRCT-1 cells 1hT72hR (1 h 5nM trabectedin treatment and 72 h after drug wash-out); lane 4, OVCAR5 cells. c EWS-WT1 chimeric structure diagram and different isoforms of EWS-WT1 chimera, in JN-DSRCT-1 cells. d EWS-WT1 variant transcripts with or without KTS domain in JN-DSRCT-1 cells