Fig. 4

Effect of FrA on mitogen-activated protein kinases (MAPK). a, b RT112 cells were treated with FrA for 1 h (a) or 48 h (b), and then protein extracts were analyzed by Western blotting. c Effect of SP600125 (a specific JNK1/2 inhibitor) on the survival of RT112 cells treated with FrA. Drugs were combined in the constant molar ratio C(FrA) : C(SP600125) = 1 : 15, and cells were cotreated with the individual drugs or their combination for 48 h. The data were generated using trypan blue-based viability assay. Cells were pretreated with SP600125 in 50 μL/well culture media for 1 h. The combinational index (CI) was calculated with the CompuSyn v.1.0. software