Fig. 3

AUY922 disruption of FANCA leads to increased dependency on ATM in response to CCRT. a Western blot analysis of RAD51 and FANCA depletion by AUY922 in the p53mt HNSCC cell lines CAL27, FaDu and HN5. b Confirmation of FANCA and ATM knockdown using lentiviral shRNA by western blot in the p53 mutant HNSCC cell line CAL27 vs scrambled shRNA. c Clonogenic survival assay showing cisplatin; radiation; or CCRT toxicity to CAL27 cells expressing scrambled (SCR) or FANCA shRNA vs control cells with no lentiviral infection. d, e Quantification of RAD51 or S1981 phospho-ATM foci by confocal microscopy in SCRsh or ATMsh CAL27 cells. CCRT and AUY922 doses as outlined in Fig. 2. f S1981 phospho-ATM foci in FaDu and HN5 cells in response to CCRT and AUY922. g Clonogenic survival assay showing cisplatin, radiation, or CCRT sensitizing effect of HSP90i by AUY922 in scrambled of ATM shRNA expressing CAL27 cells. All values mean ± SEM of at least 3 independent experiments. Statistical analysis by 2-tailed t-test; *p < 0.05, **p < 0.01, ***p < 0.001