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Fig. 2 | BMC Cancer

Fig. 2

From: Photodynamic therapy combined to cisplatin potentiates cell death responses of cervical cancer cells

Fig. 2

Fluorescence images obtained from IN Cell Analyzer, using Hoechst 33342, propidium iodide and fluorescein diacetate. Cell lines C-33 A, HaCaT and SiHa were submmited to Hoechst 33342, propidium iodide and fluorescein diacetate staining after treatments (CUR [25 μM for 6 h]; DOX [50 μg/mL for 6 h]; CisPT [41.6 μM for 6 h]; MBPDT [19.5 μM MB + 5.11 J/cm2 LED 660 nm]; MBPDT + CisPT [19.5 μM MB + 5.11 J/cm2 LED 660 nm + 1.3 μM CisPT for 6 h]; PGPDT [0.5 μM PG + 2.76 J/cm2 LED 630 nm]; CisPT + PGPDT [1.3 μM CisPT for 6 h + 0.5 μM PG + 2.76 J/cm2 LED 630 nm]). White arrows indicate representative apoptotic cells and yellow arrows indicate representative necrotic cells. 20X objective. NT: non-treated; CUR: curcumin; DOX: doxorubicin; CisPT: cisplatin; MBPDT: photodynamic therapy mediated by methylene blue; PGPDT: photodynamic therapy mediated by Photogem

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