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Fig. 3 | BMC Cancer

Fig. 3

From: The Yin/Yan of CCL2: a minor role in neutrophil anti-tumor activity in vitro but a major role on the outgrowth of metastatic breast cancer lesions in the lung in vivo

Fig. 3

Tumor cells secrete significantly higher levels of CCL2 compared to naïve and tumor entrained neutrophils. a Conditioned media was collected from cultured cells after 18-h incubation at 37 °C, and then CCL2 levels were analyzed by ELISA. Tumor cells tended to secrete higher levels of CCL2 than naïve neutrophils, but there were no statistical differences among the groups. Kruskal-Wallis test with Dunn’s test for multiple comparisons; mean ± SD are graphed. b CCR2 expression on neutrophils isolated from BALB/c versus FVB mice. Membrane expression of CCR2 was evaluated on neutrophils isolated from BALB/c and FVB mice using protocols described in Methods using PE-conjugated anti-murine CCR2. While CCR2 was expressed by only 6.03% of the neutrophils from BALB/c mice, 33.1% of the neutrophils from FVB mice expressed cell surface CCR2

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