Fig. 2

Naïve or tumor entrained neutrophils (TENs) are able to kill PyMT tumor cells in vitro, but CCL2 does not increase this effect. PyMT cultures were seeded the same as 4T1 and 67NR with the exception that neutrophils were isolated from either FVB or C57BL/6 mice, depending on cell line background. a Naïve neutrophils from FVB mice were not capable of killing tumor cells, but CCL2 addition to these naïve neutrophils significantly killed tumor cells (p = 0.005). b TENs were capable of killing tumor cells (p = 0.009). However, CCL2 did not significantly increase killing (p = 0.5). c Naïve neutrophils from C57BL/6 mice did not kill C57BL/6 PyMT cells (p = 0.058), and CCL2 addition to this co-culture enhanced the number of viable PyMT tumor cells (p < 0.001). d C57BL/6 TEN had little effect on the viability of autologous PyMT tumor cells, but CCL2 addition to these TENs resulted in a modest reduction in viable PyMT tumor cells (p = 0.005). Kruskal-Wallis test with Dunn’s test for multiple comparisons. Values are graphed as mean ± SD