Fig. 2

Genomic analyses. a Heat map for the mutations in each cancer component and ctDNA detected in plasma. Column next to heatmap shows the mutated genes and amino acid changes. AF, allele fraction b Validation of somatic mutations by Sanger sequencing. Representative image of read alignments visualized with IGV of targeted sequencing data (left) and Sanger sequencing data (right). The arrow shows the position of the variant. Well, well-differentiated adenocarcinoma; Poor, poorly differentiated adenocarcinoma; Sarco, sarcomatoid carcinoma