Fig. 6

Knockdown of STK11 and PRKAA2 downregulates gastrin mediated autophagy. (a) Knockdown of STK11 in AGS-Gr cells. (b) AGS-Gr cells transfected with siRNA STK11 for 48 h before BafA1 and gastrin treatment (4 h). Expression of SQSTM1 and MAP1LC3B-II are shown by immunoblotting representing one of four independent experiments. Bar graphs (a and b) show mean +/− SEM (n = 3, P- value ** ≤ 0.02 and * ≤ 0.05). (c) Knockdown of PRKAA2 in AGS-Gr cells. (d) AGS-Gr cells transfected with siRNA PRKAA2 and treated with BafA1 and gastrin (4 h). Expression of PRKAA2 and SQSTM1 is shown by immunoblotting representing one of three independent experiments. (e) Gastrin induced migration is dependent on PRKAA2. AGS-Gr cells treated with gastrin (1 nM) and PRKAA2 inhibitor Compound C (10 μM) for 24 h. Migration was monitored using xCELLigence technology. Untreated (red), Comp C (blue), gastrin + Comp C (pink), gastrin (green). Bar graphs (a, b, c, d and e) show mean +/− SEM (n = 3, P-value*: ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001)