Fig. 3

Collagen-induced activation and pharmacologic inhibition of DDR1 in vitro. a DDR1 and phosphorylated DDR1 levels in various gastric cancer cell lines were determined by Western blotting. b KATO-III and MKN28 cells were stimulated with rat tail type I collagen. Lysates were probed for indicated proteins by Western blotting. c KATO-III and MKN28 cells plated on collagen-coated dishes were treated with 7rh benzamide (0.18, 0.54, and 1.62 μM). The level of phosphorylated DDR1 and PYK2, E-cadherin, and β-actin were determined by Western blotting. d MKN28 cell morphology was changed into a more linear and mesenchymal-like shape by collagen stimulation, and this altered morphology was partially inhibited by 7rh benzamide treatment. The representative images are shown