Fig. 6
From: Lung cells support osteosarcoma cell migration and survival
ALDH is involved in the increased migration of Osteosarcoma cells induced by HULEC-5a cell and CM. a ALDH mRNA level in SaOS2 and SJSA-1 cells is increased in HULEC-5a CM compared with regular medium. b ALP expression in SaOS2 and SJSA-1 cell was suppressed by ALDH inhibitor disulfiram in a dose-dependent manner, and HULEC-5a cell and CM rescue the ALP expression which is inhibited by disulfiram. Quantitative densitometry assays for ALP staining in Fig. 6b. * indicates the significant difference of the ALP expression in SaOS2 and SJSA-1 cells between single cell type culture of SaOS2/or SJSA-1 and the co-culture of SaOS2 and HULEC-5a cell treated with indicated dose of disulfiram. # indicates the significant difference of the ALP expression between regular medium and HULEC-5a CM in SJSA-1 cells treated with indicated dose of disulfiram. c BrdU staining for the SaOS2 and SJSA-1 cells treated with/without disulfiram. Brown color represents the positive staining