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Fig. 4 | BMC Cancer

Fig. 4

From: Lung cells support osteosarcoma cell migration and survival

Fig. 4

Osteosarcoma cell ALP expression increased in the co-culture of HULEC-5a cell. a SaOS2, SJSA-1 and U2OS cells were co-cultured with HULEC-5a cells for 24, 48 and 72 h, followed by ALP staining assay. Single cell type culture of SaOS2, SJSA-1, U2OS and HULEC-5a were used as control. b Quantitative densitometry assays for ALP staining in Fig. 4a. c High magnitude (100x) photos of ALP staining in Fig. 4a. d SaOS2, SJSA-1 and U2OS cells were cultured in regular medium or HULEC-5a CM for 72 h, followed by ALP staining. e Quantitative densitometry assays for ALP staining in Fig. 4d. * indicates the significant change of the ALP expression between SaOS2 and SaOS2 and HULEC-5a cell co-culture; # indicates the significant change of the ALP expression between SJSA-1 and SJSA-1 and HULEC-5a cell co-culture. f) High magnitude (100x) photos of ALP staining in Fig. 4d

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