Fig. 1

Osteosarcoma cell migration increased in lung cell conditioned medium (CM). a Osteosarcoma cells migration in HULEC-5a and MRC5 cell CM using transwell co-culture, NIH3T3 cell CM and cell culture medium (containing 10% FBS) are used as control. 600 μl CM was loaded to bottom chamber of 24-well plate and 2x10⁴ SaOS2, SJSA-1 and U2OS cells in 100 μl serum free medium were added to the top insert of transwell. Top insert containing OS cells was put back were cultured in CM for 48 h, followed by counting the number of migrated cells. b Osteosarcoma cells migration in HULEC-5A and MRC5 cell CM using xCELLigence system