Fig. 4
From: Sensitivity of osteosarcoma cells to HDAC inhibitor AR-42 mediated apoptosis
HDAC inhibition causes dose-dependent decreases in Akt phosphorylation and multiple downstream signaling molecules. D17 and SAOS-2 OS cells were exposed to the indicated concentrations of AR-42, SAHA, and DMSO for 48 h. Protein lysates were generated and separated by SDS-PAGE and Western blotting was performed with the indicated antibodies. Representative immunoblots showing dose-dependent effects of AR-42 and SAHA on Akt and GSK3β phosphorylation and survivin. β-Actin was used as a loading control. Protein lysates of similarly treated cells were immunoblotted with mTOR and p70S6K phospho-antibodies