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Fig. 8 | BMC Cancer

Fig. 8

From: Cryptotanshinone activates AMPK-TSC2 axis leading to inhibition of mTORC1 signaling in cancer cells

Fig. 8

Activation of TSC2 involved in CPT inhibition of mTORC1 signaling. a Serum-starved Rh30 and DU145 cells were firstly exposed to CPT (0-20 μmol/L) for 2 h, then stimulated with (10 ng/mL) for 1 h, followed by western blotting assay for proteins expression. Rh30 cells respectively transfected with sh-TSC2 or sh-GFP were: (b) seeded in 96-well plates (104 cells/well) respectively and then followed by cell proliferation assay; (c) starved for serum and pretreated with CPT (0-20 μmol/L) for 2 h, and then stimulated with (10 ng/mL) for 1 h, followed by western blotting assay for proteins expression. d Co-immunoprecipitation assay was executed for TSC2 and TSC1. Note: IgG-HC, heavy chain of IgG

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