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Fig. 3 | BMC Cancer

Fig. 3

From: Cryptotanshinone activates AMPK-TSC2 axis leading to inhibition of mTORC1 signaling in cancer cells

Fig. 3

CPT does not affect mTORC1 formation. Serum-starved Rh30 cells were firstly exposed to CPT (0-20 μmol/L) for 2 h, then stimulated with IGF-1 (10 ng/mL) for 1 h, followed by western blotting assay for the indicated proteins expression (a, c), or by immunoprecipitation with antibody to mTOR plus protein A/G agarose, and immunoblotting with antibodies to mTOR, raptor, rictor and mLST8 (b)

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