Fig. 8

Ubiquitous, but not endothelial-specific, loss of Dll4 promotes intestinal differentiation towards the secretory lineages. a, b, d, e Immunofluorescence stainings of small (a, d) and large (b, e) intestinal tumor cryosections (10 μm) from Apc ^Min/+ endoDll4 ^-/- and ubiqDll4 ^-/- mice versus controls (CT) at 18 weeks of age. Representative images of staining density for E-cadherin (in green) (a, b) and lysozyme (in green) (d, e). Nuclei were counterstained with DAPI (in blue). Scale bars = 100 μm. c, f Graphic bars represent the tumor relative (%) ± SEM density of E-cadherin (c) and lysozyme (f) in the small (SI) and large (LI) intestinal tumors from the animals described above. One experiment with n = 6 per group and 6 fields per animal. g, h PAS staining (of goblet cells) of paraffin-embedded small (g) and large (h) intestinal tumor sections (4 μm) from Apc ^Min/+ endoDll4 ^-/- and ubiqDll4 ^-/- mice versus controls (CT) at 18 weeks of age. Scale bars = 100 μm. i, j Graphic bars represent the relative proportion (%) ± SEM of goblet cells in the small (i) and large (j) intestinal tumor epithelium from the animals described above. One experiment with n = 6 per group and 6 fields per animal. k, l RT-PCR analysis of Akp3, Muc2, Lyz, Neurog3, Atoh1, Klf4 relative expression in the Apc ^Min/+ endoDll4 ^-/- and Apc ^Min/+ ubiqDll4 ^-/- small (k) and large (l) intestinal tumor samples. One experiment with n = 3 per group. *P < 0.05; **P < 0.01