Fig. 5

Endothelial-specific and ubiquitous Dll4 deregulation affects similarly the Apc ^Min/+ tumor angiogenesis. a, b, e, f, h, i Immunofluorescence stainings of 20 μm small (a, e, h) and large (b, f, i) intestinal tumor cryosections from Apc ^Min/+ endoDll4 ^-/-, Apc ^Min/+ubiqDll4 ^-/- mice and controls (CT) at 18 weeks of age. Scale bars = 100 μm. Representative images of staining density for PECAM-1 (in green) and α-SMA (in red) (a, b), for lectin (in green) and PECAM-1 (in red) (e, f), and for PECAM-1 (in green) and Evans blue (in red) (h, i). The nuclei were counterstained with DAPI (in blue). c, d, g, j Graphic bars represent the relative (%) ± SEM small (SI) and large (LI) intestinal tumor vascular density (c), maturity (d), perfusion (g) and extravasation (j) in the animals described above. One experiment with n = 6 per group and 6 fields per animal. *P < 0.05; **P < 0.01