Fig. 3

Hes1 and Hes5 expression pattern in the small and large intestine and in Apc ^Min/+ intestinal adenomas. a-l Immunohistochemical analysis of Notch effectors Hes1 and Hes5 expression (indicated by an arrow) in three series of sequential sections of the normal small and large intestine of C57BL/6 mice, and in Apc ^Min/+ small and large intestinal adenomas (n = 2 per group, and 2 sections per slide). Hes1 (a-c) and Hes5 (d-f) expression in the small intestinal crypts (a, d), villi (b, e) and adenomas (c, f). Hes1 (g-i) and Hes5 (j-l) expression in the large intestine in the bottom (g, j) and top (h, k) of the crypts and in adenomas (i, l). Scale bar = 20 μm. m-n Graphic bars represent the average staining score ± SEM for each protein in the normal tissue and adenomas of the small intestine (SI) (m) and large intestine (LI) (n). *P < 0.05; **P < 0.01