Fig. 2

Notch pathway expression pattern in the large intestine and in Apc ^Min/+ large intestinal adenomas. a-g Immunohistochemical analysis of Notch ligands and receptors expression (indicated by a red arrow) in three series of sequential sections of the normal large intestine of C57Bl/6 mice, and in Apc ^Min/+ large intestinal adenomas (n = 2 per group, and 2 sections per slide). Control staining was performed with the specific species IgG. Notch ligands (a-c) and receptors (d-f) expression in the large intestine in the bottom (a, d) and top (b, e) of the crypts and in adenomas (c, f). Scale bar = 20 μm. Red arrow points to a colonocyte (b); Yellow arrow indicates a goblet cell (b); Orange arrow in (c) indicates the tumor epithelium; LP lamina propria, S stroma. g Graphic bars represent the average staining score ± SEM for each protein in the normal large intestine (LI) and adenomas of the same region. *P < 0.05; **P < 0.01