Fig. 3

Apoptotic effects of BT-cisplatin combination on isogenic pairs of ovarian cancer cell lines. Representative images of Hoechst 33258 staining of A2780 and A2780-CDDP (a) or IGROV and IGROV-1-CDDP (c) cells treated with BT and/or cisplatin as indicated. Percent of apoptosis in terms of DNA fragmentation (quantified via TUNEL assay) are shown for A2780 and A2780-CDDP (b) or IGROV and IGROV-1-CDDP (d) cells treated with BT or cisplatin alone or in combination. Data were expressed as means ± SD of duplicate experiments. Comparisons between cisplatin alone treated and combination treated for each cell line were performed using Student’s t–test. Asterisks (*) indicate p < 0.05