Fig. 2
Vaccination of LMP1/pcDNA3.1 suppresses LMP1-expressing tumor growth in vivo. a LMP1/pcDNA3.1 vaccine construct. Restriction sites are as indicated. CRT, calreticulin. b Effect of vaccination on LMP1-expressing tumor growth in vivo. Upper panel, schedule of tumor injection and vaccination. Lower panel, tumor growth curves. *, P < 0.05. C57BL/6 J mice were subcutaneously injected with 1 × 105 LMP1-expressing TC-l cells (D0). Vaccination with either empty pcDNA3.1 (control) or LMP1/pcDNA3.1 (vaccine) (n = 3) were given weekly on the 7th day after tumor injection for 3 consecutive weeks. Tumor volumes were measured every 3 days and the mice were sacrificed on the fourth week