Fig. 5

Effects of C3L5-CM, PGE2 and PGE1OH on molecular profile of RMLEC. a Relative mRNA level of COX-2, EP4, VEGF-C, VEGF-D in RMLEC treated with C3L5 -CM for 24 h compared to control. b Western blots with cytoplasmic extracts obtained from above treated cells showing the expression of EP4, COX-2, VEGF-C, VEGF-D at the protein level. c Cytoplasmic PGE2 level (measured by ELISA) in C3L5-CM treated RMLEC compared to control. d Relative COX-2, VEGF-D and EP4 mRNA expression (normalized to GAPDH) in untreated (control) or PGE2 and PGE1OH treated RMLEC. e Western blots of COX-2, VEGF-D and EP4 protein in untreated or PGE2 and PGE1OH treated RMLEC. Error bar indicates mean ± SEM. (*) and (**) indicates p values < 0.05 and <0.001 respectively