Fig. 4

Effects of EP4 knock-down on migration and tube formation by RMLEC. a Real-time qPCR of EP4 mRNA expression (normalized to GAPDH) in EP4 knock-down RMLEC. b Cellular migration of EP4 KD RMLEC in response to PGE2 (20μM), EP4 agonist PGE1OH (20μM), and 24h C3L5-CM. c Tube formation by EP4 KD RMLEC on GFR-Matrigel at 24 h in the presence of the stimulating agents at same concentrations used for migration assay. Bars equal 100 μm. d Quantification of the number of branching points in the captured area at 10 different FOV for each experimental condition as determined by imageJ. Error bar represents mean ± SEM of three different experiments. (*) and (**) represent p < 0.05 and p < 0.001 respectively